abstract：:Skin fibroblasts from patients with inherited adenomatosis of the large bowel (ACR-SF) possess alterations in actin microfilament (MF) organization which serve to distinguish "predisposed" cells from fibroblasts derived from normal individuals (NSF). MF bundle frequency and diameter were considerably reduced in ACR-SF...

journal_title：Experimental cell research

authors： Higgins PJ,Kopelovich L

更新日期：1991-08-01 00:00:00

abstract：:Periosteal cells were enzymatically liberated from the tibiae of young chicks, introduced into cell culture, and allowed to reach confluence. The morphology of the cells gave the impression of a relatively homogeneous population of fibroblast-like cells. These cultured cells did not overtly express osteogenic or chond...

journal_title：Experimental cell research

authors： Nakahara H,Dennis JE,Bruder SP,Haynesworth SE,Lennon DP,Caplan AI

更新日期：1991-08-01 00:00:00

abstract：:Although 12-day-old chick embryo retinal pigment epithelial (RPE) cells in situ do not express the proliferating cell nuclear antigen (PCNA) which is known to function as an auxiliary protein of DNA polymerase delta, they do so when cultured on glass. Conversely, PCNA was not expressed by RPE cells of the same age mai...

journal_title：Experimental cell research

authors： Hergott GJ,Kalnins VI

更新日期：1991-08-01 00:00:00

abstract：:We have shown previously that there is a good correlation between the degree of microtubule disassembly by methylmercury (MeHg) and the extent of inhibition of DNA replication in Concanavalin A (Con A)-stimulated mouse splenic lymphocytes. The purpose of this study was to determine if these two events are causally rel...

journal_title：Experimental cell research

authors： Roy C,Prasad KV,Reuhl KR,Little JE,Valentine BK,Brown DL

更新日期：1991-08-01 00:00:00

abstract：:The base at the first anticodon ("wobble") position of certain eukaryotic tRNA species is either guanine or the hypermodified base queuine. These tRNA species are synthesized with guanine in the wobble position (tRNAG); this guanine can then be replaced with queuine by the action of the enzyme tRNA-guanine ribosyltran...

journal_title：Experimental cell research

authors： Parniak MA,Andrejchyshyn S,Marx S,Kleiman L

更新日期：1991-07-01 00:00:00

abstract：:The expression of the "erythroid/brain" type glucose transporter (GLUT1) seems to be a feature of "barrier" tissues, at least in humans. Recently, we reported that GLUT1 is highly expressed in the basal layers of either "authentic" human epidermis or human epidermis reconstituted in culture and that its expression see...

journal_title：Experimental cell research

authors： Gherzi R,Melioli G,De Luca M,D'Agostino A,Guastella M,Traverso CE,D'Anna F,Franzi AT,Cancedda R

更新日期：1991-07-01 00:00:00

abstract：:Xenopus oocytes carry IGF-I receptors, and undergo meiotic maturation in response to binding of IGF-I or insulin to the IGF-I receptor. Maturation is initiated upon activation of the IGF-I receptor tyrosine kinase and requires tyrosine dephosphorylation of p34cdc2, the kinase component of maturation promoting factor (...

journal_title：Experimental cell research

authors： Hainaut P,Giorgetti S,Kowalski A,Ballotti R,Van Obberghen E

更新日期：1991-07-01 00:00:00

abstract：:Ultraviolet (UV) irradiation of cultured human skin fibroblasts causes the disassembly of their microtubules. Using indirect immunofluorescence microscopy, we have now investigated whether damage to the microtubule precursor pool may contribute to the disruption of microtubules. Exposure to polychromatic UV radiation ...

journal_title：Experimental cell research

authors： Zamansky GB,Perrino BA,Chou IN

更新日期：1991-07-01 00:00:00

abstract：:The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...

journal_title：Experimental cell research

authors： Yaoi Y,Hashimoto K,Takahara K,Kato I

更新日期：1991-06-01 00:00:00

abstract：:In this study we examined by Northern blot analysis the expression of Raf-1 protooncogene in normal human peripheral blood leukocytes. Unlike thymocytes, circulating lymphocytes did not express appreciable levels of Raf-1 mRNA. In contrast, polymorphonuclear cells (PMN) had high levels of Raf-1 transcripts. Also densi...

journal_title：Experimental cell research

authors： Colotta F,Polentarutti N,Mantovani A

更新日期：1991-06-01 00:00:00

abstract：:Rat peritoneal mast cells (RPMC) and rat basophilic leukemia (RBL) cells are representative of connective tissue-type (CTMC) and mucosal-type (MMC) mast cells, respectively. Using polyethylene glycol, we have fused RPMC with 6-thioguanine resistant, HAT (hypoxanthine, aminopterin, thymidine) sensitive RBL-CA10.7 or RB...

journal_title：Experimental cell research

authors： Zheng YL,Chan BM,Rector ES,Berczi I,Froese A

更新日期：1991-06-01 00:00:00

abstract：:The effects of hormonal promotion of T24-ras oncogene-transfected rat embryo fibroblasts (REF) were compared to cotransformation of these cells with adenovirus E1A and ras. Cotransfection of E1A + ras resulted in the appearance of morphologically transformed cells which were very efficiently established into cell line...

journal_title：Experimental cell research

authors： Marshall H,Martens I,Svensson C,Akusjärvi G,Linder S

更新日期：1991-05-01 00:00:00

abstract：:Primary cultures of renal rabbit proximal tubule cells were initiated from a pure suspension of proximal tubule fragments. Proximal tubule cells were grown in a hormone-supplemented, serum-free medium containing low concentrations of antibiotics. Confluent monolayers exhibited multicellular dome formation, indicating ...

journal_title：Experimental cell research

authors： Toutain H,Vauclin-Jacques N,Fillastre JP,Morin JP

更新日期：1991-05-01 00:00:00

abstract：:Exponentially growing Chinese hamster V79-171b lung fibroblasts seeded at high density on plastic (approximately 7 x 10(3) cells/cm2) flatten, elongate, and produce significant amounts of extracellular fibronectin. When lysed in weak alkali/high salt, the rate of DNA denaturation following exposure to ionizing radiati...

journal_title：Experimental cell research

authors： Olive PL,Vanderbyl S,MacPhail SH

更新日期：1991-04-01 00:00:00

abstract：:The endothelial cell lining of blood vessels is now recognized as an active interface between blood and the underlying tissue. Modulation of cholesterol levels in several cell types has resulted in altered cell function. We have removed cholesterol from the endothelial cell membrane and have observed corresponding alt...

journal_title：Experimental cell research

authors： Broadley C,Dawidowicz E,Chong PL,Hoover R

更新日期：1991-03-01 00:00:00

abstract：:Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein which is involved in activity of the pronuclei during conjugation (O. Numata, T. Sugai, and Y. Watanabe (1985) Nature (London) 314, 192-194). Using monoclonal and polyclonal antibodies, we here demonstrate the presence of a cross-reactive...

journal_title：Experimental cell research

authors： Numata O,Tomiyoshi T,Kurasawa Y,Fu ZX,Takahashi M,Kosaka T,Chiba J,Watanabe Y

更新日期：1991-03-01 00:00:00

abstract：:We have searched for brain-specific extracellular molecules using a library of monoclonal antibodies against surface antigens of differentiated PC12h cells. One of the monoclonal antibodies, PCH42-14, recognized a 27/26-kDa protein of 10-week-old rat brain on immunoblotting. PCH42-14 antigen was detected only in brain...

journal_title：Experimental cell research

authors： Ishikawa M,Kuroda Y,Kobayashi K,Sawada H,Hayashi M

更新日期：1991-03-01 00:00:00

abstract：:The DNA topoisomerase I inhibitor camptothecin (CAM) is selectively cytotoxic to S-phase cells of HL-60, and some other myelogenous leukemic lines. The early effects of cell exposure to 0.05-0.2 micrograms/ml CAM are seen after 2 h; at that time a progressive degradation of DNA in the chromatin of S-phase cells is ini...

journal_title：Experimental cell research

authors： Del Bino G,Lassota P,Darzynkiewicz Z

更新日期：1991-03-01 00:00:00

abstract：:We have studied the cytotoxicity of ricin in cells treated with brefeldin A (BFA), which dramatically disrupts the structure of the Golgi apparatus causing Golgi content and membrane to redistribute to the ER. BFA inhibits the cytotoxicity of ricin in Chinese hamster ovary, normal rat kidney, and Vero cells and abolis...

journal_title：Experimental cell research

authors： Yoshida T,Chen CC,Zhang MS,Wu HC

更新日期：1991-02-01 00:00:00

abstract：:Indirect immunofluorescence using anti-human placental bFGF antibodies demonstrates the presence of bFGF-like reactivity in the cytoplasm and in the nucleus of adult bovine aortic endothelial cells and of normal and transformed fetal bovine aortic endothelial AG 7680 and GM 7372 cells. Biologically active immunoreacti...

journal_title：Experimental cell research

authors： Dell'Era P,Presta M,Ragnotti G

更新日期：1991-02-01 00:00:00

abstract：:In DNA isolated from proliferating human lymphoblastoid CCRF-CEM cells which had been pulse-labeled by exposure to [3H]thymidine for periods from 30 s to 10 min, single-stranded regions were analyzed by caffeine-gradient elution from benzoylated DEAE-cellulose. Two classes of structural defect were evident. Some repli...

journal_title：Experimental cell research

authors： Stewart BW,Kavallaris M,Catchpoole D,Norris MD

更新日期：1991-02-01 00:00:00

abstract：:Previous hybrid studies involving fusion of normal with immortal human cells indicated that the phenotype of cellular senescence is dominant and that immortality results from recessive changes in normal growth regulatory genes. We have further assigned 28 different immortal human cell lines to at least four complement...

journal_title：Experimental cell research

authors： Ning Y,Shay JW,Lovell M,Taylor L,Ledbetter DH,Pereira-Smith OM

更新日期：1991-01-01 00:00:00

abstract：:To determine which endocytic compartments are sensitive to sucrose-induced osmotic swelling, CHO and Vero cells were cultured for 1-3 days in media containing 0.03 to 0.05 M sucrose. (Sucrose is internalized but not digested by these cells.) To immunolocalize late endocytic compartments, cells were fixed with formalde...

journal_title：Experimental cell research

authors： DeCourcy K,Storrie B

更新日期：1991-01-01 00:00:00

abstract：:We report that a specific inhibitor of types 1 and 2A phosphatases, okadaic acid (OA), induces germinal vesicle break down (GVBD) and chromosome condensation when microinjected into denuded mouse oocytes maintained in prophase block by analogs of cAMP, inhibitors of phosphodiesterase, or a tumor-promoting phorbol este...

journal_title：Experimental cell research

authors： Gavin AC,Tsukitani Y,Schorderet-Slatkine S

更新日期：1991-01-01 00:00:00

abstract：:Specific cholecystokinin (CCK) receptor and gastrin receptor antagonists were used to assess what role, if any, these receptors have in autocrine cell growth. Although the cholecystokinin receptor antagonist, L-364,718, inhibited cell proliferation in a broad spectrum of cell lines, the gastrin antagonist, L-365,260, ...

journal_title：Experimental cell research

authors： Thumwood CM,Hong J,Baldwin GS

更新日期：1991-01-01 00:00:00

abstract：:Trophoblast cells were grown on filters that allow access to apical and basal surfaces of cells. Using this experimental system, IgG transport was shown to be specific and to occur primarily in the apical to basal direction. This transport was time- and temperature-dependent, with approximately 10% of added IgG appear...

journal_title：Experimental cell research

authors： Sooranna SR,Contractor SF

更新日期：1991-01-01 00:00:00

abstract：:After the 10th nuclear cycle the yolk centrosomes follow an irregular pathway. Unlike the somatic centrosomes, which move to the opposite poles of the nuclei to form the bipolar spindles, the yolk centrosomes remain as pairs at one pole of the yolk nuclei or shift feebly and nucleate irregular spindles, most of which ...

journal_title：Experimental cell research

authors： Callaini G,Dallai R

更新日期：1991-01-01 00:00:00

abstract：:We present the first evidence that secretory organelle docking at the cell membrane can be reversed in vivo. In nondischarge (nd) mutants of Paramecium tetraurelia all trichocysts can be detached from the cell surface within 2-3 h by different means, including cytochalasin B (but not D), high cell density, or Ca2+ ion...

journal_title：Experimental cell research

authors： Pape R,Plattner H

更新日期：1990-12-01 00:00:00

abstract：:Cellular senescence is characterized by a decline in sensitivity to growth factors resulting in cessation of cellular growth. The expression of cellular or viral oncogenes may result in the establishment of cell lines with unlimited proliferative potential ("immortalization"). A variety of viral and cellular oncogenes...

journal_title：Experimental cell research

authors： Linder S,Marshall H

更新日期：1990-11-01 00:00:00

abstract：:Changes in the expression pattern of DNA polymerase beta gene during rat lung, brain, and testis development have been investigated. A decrease in the level of beta-pol mRNA was observed during postnatal development of lung and brain. By contrast, an almost 20-fold increase in the level of beta-pol mRNA was observed d...

journal_title：Experimental cell research

authors： Nowak R,Woszczynski M,Siedlecki JA

更新日期：1990-11-01 00:00:00

abstract：:The nucleolar precursor bodies (NPBs) are electrondense, homogeneous, and finely filamentous intranuclear structures occurring in mammalian zygotes during the early postfertilization development and subsequently transforming into nucleoli. In this study we addressed the question if the assembly of the NPBs is correlat...

journal_title：Experimental cell research

authors： Tesarik J,Kopecny V

更新日期：1990-11-01 00:00:00

abstract：:Interleukin 3 (IL-3) stimulates the growth of various types of hemopoietic progenitors. In vitro, survival of a series of murine cell lines derived from either neoplastic or nonneoplastic hemopoietic tissue shows a strict IL-3 dependence. In order to test the implication of energy metabolism in this dependence as clai...

journal_title：Experimental cell research

authors： Reisbach G,Ellwart J,Dörmer P

更新日期：1990-10-01 00:00:00

abstract：:A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...

journal_title：Experimental cell research

authors： Borrione AC,Zanellato AM,Giuriato L,Scannapieco G,Pauletto P,Sartore S

更新日期：1990-09-01 00:00:00

abstract：:We have studied the mechanisms of angiotensin II (A-II) transport through a cultured arterial endothelial cell monolayer. The transport of 125I-labeled A-II was inhibited by excess unlabeled A-II (50 microM) and [Sar1, Ile8]-A-II (50 microM), but was not inhibited by bradykinin (50 microM). The transport process was s...

journal_title：Experimental cell research

authors： Mineo C,Yagyu Y,Imanaka T,Takano T

更新日期：1990-09-01 00:00:00

abstract：:In the past, a unique type of precursor for a secretory protein was discovered. It contains a central repetitive domain rich in threonine residues and terminal cysteine-rich domains. Due to striking homologies of these terminal domains with pancreatic spasmolytic polypeptide, originally the name "prepro-spasmolysin" w...

journal_title：Experimental cell research

authors： Hauser F,Gertzen EM,Hoffmann W

更新日期：1990-08-01 00:00:00

abstract：:Primary cultures of human keratinocytes form a multilayered tissue. By incubating the tissue cultures in Ca2(+)-free medium the differentiated cell layers can be stripped off leaving a basal cell monolayer. We have developed a method for high-frequency transfection of these epidermal basal cells with genes inserted in...

journal_title：Experimental cell research

authors： Jensen TG,Jensen PK,Nørgård JO,Bolund L

更新日期：1990-08-01 00:00:00

abstract：:Liver cells of new-born rats, which were found to be able to form spheroidal aggregates when cultured on a nonadherent plastic substratum, were studied under various conditions of culture, mainly by adding different nutrients and growth factors to the culture medium. Analysis of hepatocyte-specific functions was carri...

journal_title：Experimental cell research

authors： Tong JZ,Bernard O,Alvarez F

更新日期：1990-07-01 00:00:00

abstract：:Tyrosine-specific protein kinase activity of pp60c-src was examined in human Y79 retinoblastoma cells cultured in monolayers after clusters in suspension culture had been dissociated. The activity increased five- to six-fold between Days 1 and 7 in the monolayer cultures, with a concomitant increase in numbers of cell...

journal_title：Experimental cell research

authors： Kato G

更新日期：1990-07-01 00:00:00

abstract：:The characteristics underlying cytosolic free calcium oscillation were evaluated by superfused dual wave-length microspectrofluorometry of fura-2-loaded single acinar cells from rat pancreas. Application of a physiological concentration of cholecystokinin octapeptide (CCK) (20 pM) induced a small basal increase in cyt...

journal_title：Experimental cell research

authors： Tsunoda Y

更新日期：1990-06-01 00:00:00

abstract：:Basic fibroblast growth factor (bFGF) binding to ocular tissues has been studied by autoradiographical and biochemical approaches directly performed on sections during mouse embryonic and postnatal development. Frozen sections of embryos (9 to 18 days), newborns, and adults (1 day to 6 months) were incubated with iodi...

journal_title：Experimental cell research

authors： Fayein NA,Courtois Y,Jeanny JC

更新日期：1990-05-01 00:00:00